The program BINMATNEST calculates the nestedness temperature of binary presence-absence matrices. You can download from this page both the program and some sort of User's Manual. The use of this program is completely free. Whenever used, it should be cited as:

Rodríguez-Gironés, M.A., & Santamaría, L. (2010). How foraging behaviour and resource partitioning can drive the evolution of flowers and the structure of pollination networks. Open Ecology Journal, 3: 1-11.